Read e-book online Advanced Techniques in Biological Electron Microscopy II: PDF

By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)

ISBN-10: 3642668097

ISBN-13: 9783642668098

ISBN-10: 3642668119

ISBN-13: 9783642668111

The use of the time period "advanced" within the identify of this e-book is slightly ar­ bitrary and extremely a lot relative with recognize to time. Many recommendations which have been thought of on the "cutting side" of ultrastructural technique quite a few years in the past at the moment are rou tin ely utilized in a variety of laboratories. it is easy to cite freeze-fracture, cryothin sectioning, or certainly lots of the box of test­ ning electron microscopy as concrete examples. hence using the time period "ad­ vanced strategies" has to be interpreted with reference to the current state-of-the-art, and turns out to be useful merely in informing the capability reader that this quantity isn't a primer for use as an preliminary advent into uncomplicated organic elec­ tron microscopy. many glorious volumes have crammed that area of interest some time past few years, and it's not meant that this modest booklet be an entire com­ pendium of the sector. additionally, any constrained choice of papers on advanc­ ed thoughts unavoidably displays the personal tastes and arbitrary whims of the editor, thereby apart from many both very important approaches which the a professional reader will easily determine. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated ideas which have been concept to symbolize complex and but ba­ sically morphological equipment for gaining elevated ultrastructural informa­ tion from organic specimens. the current quantity, nonetheless, stresses ideas which offer particular physicochemical information at the speci­ mens as well as the structural information.

Show description

Read Online or Download Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes PDF

Best techniques books

New PDF release: RNA Interference Techniques

Non-coding, inhibitory microRNAs have emerged as very important modulators of mobile gene expression, via a method known as RNA interference (RNAi). thus far, countless numbers of conserved and species-specific microRNAs were pointed out in organisms starting from single-celled algae to people. lots of those tiny RNAs at the moment are identified to play basic roles in developmental biology and illness pathogenesis.

Read e-book online Math3521: Algebraic techniques in Number Theory: Chapters PDF

Those direction notes have been written in the course of April and should of 2008, for the second one 1/2 the 3rd-year path MATH3521 Algebraic innovations in quantity thought on the college of arithmetic and statistics of the college of latest South Wales, Sydney, Australia. The notes have been written in TEX utilizing a really great format and set of macros via David Angell.

Download e-book for iPad: Models for Parallel and Distributed Computation: Theory, by Michel Cosnard (auth.), Ricardo Corrêa, Inês Dutra, Mario

Parallel and dispensed computation has been gaining a good lot of realization within the final many years. in this interval, the advances attained in computing and conversation applied sciences, and the relief within the bills of these technolo­ gies, performed a critical function within the fast development of the curiosity within the use of parallel and allotted computation in a few parts of engineering and sciences.

Download PDF by J. Gram, J. Jespersen (auth.), J. Jespersen, R. M. Bertina,: Laboratory Techniques in Thrombosis — a Manual

The 1st variation of this guide seemed in 1992 and used to be entitled ECAT Assay tactics. It was once the results of a distinct cooperation among specialists introduced jointly via the eu Concerted motion on Thrombosis and Disabilities (ECAT). The Concerted motion used to be at the moment below the auspices of the fee of the eu Union.

Extra resources for Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes

Example text

Nature (New BioI) 244, 278 - 279 (1973) McFarlane, A. : Efficient trace-labelling of proteins with iodine. Nature (London) 182,53 (1958) Miller, J. , Kingdon, H. : Phytohemagglutinin mitogenic proteins. Structural evidence for a family of isomitogenic proteins. J. Exp. Med. 138,939 - 951 (1973) Miller, J. , Boyd, W. C: Immunochemical studies of lectins. I. Partial purification of the hemagglutinating protein of Sophora Japonica. Vox Sang. : Immunocyte triggering. Cell. Immunol. 1,573 - 582 (1970) Nicolson, G.

Science 184, 1294 -1296 (1974) Nicolson, G. ]. Cell BioI. : Lymphocyte transformation induced by concanavalin A and its reversion by methyl-alpha-D-mannopyranoside. Biochim. Biophys. , Barondes, S. : Agglutinin from Limulus polyphemus: purification with formalinized horse erythrocytes as the affinity adsorbent. Biochim. Biophys. Acta 393, 115 - 123 (1975) Oikawa, T, Nicolson, G. : Inhibition of hamster egg fertilization by phytoagglutinins. Exptl. Cell Res. , Nicolson, G. : Wheat germ agglutinin blocks mammalian fertilization.

Excess dextran-iron complex is removed by three washes as before, and the samples are postfixed in 1% osmium tetroxide in phosphate buffer for 1 h and dehydrated and embedded using standard procedures. The dextran-iron particles appear in a variety of sizes but are sufficiently electron dense to allow high resolution localization. The complex does not penetrate fixed cells, and controls without lectin are unlabeled, indicating absence of nonspecific binding to cell surfaces. 2. Lectin-Mannan-Iron Complexes ROTH and FRANZ (1975) utilized a purified yeast mannan to make mannan-iron complexes (see Sect.

Download PDF sample

Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes by G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)


by Steven
4.4

Rated 4.53 of 5 – based on 37 votes