By O. Hoffmann-Ostenhof
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Extra resources for Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry
B o t t o m l e y , Preparation of a d e n o s i n e n u c l e o t i d e d e r i v a t i v e s s u i t a b l e for affinity c h r o m a t o g r a p h y , B i o c h e m . J . 139, 609 (1974). (9) H . S c h a l l e r , X . N i i u a a l w i n , F . J . B o n h o e f f e r , C . K u r z & I. N i e t z c h m a n n , Affinity C h r o m a t o g r a p h y of D N A - b i n d i n g e n z y m e s on s i n g l e - s t r a n d e d D N A - a g a r o s e c o l u m n s , E u r . J . B i o c h e m . 26, 474 (1972).
We have attached the same ligands to the pellicular support Matrex 201R (Table 1 ) . The K A is seen to be raised in all instances, by at least an order of magnitude. These measure ments were made under conditions of extremely low flow rates and with closely similar ligand concentrations. When the flow rate of these columns is increased, and the behaviour of the gel (Sepharose) based columns compared with that of the pellicular (Matrex) equivalent then the diffusional effect can be seen to be increasingly important.
Cuatrecasas & C. B. Anfinsen, Affinity Chromatography, Ann. Rev. Biochem. 40, 259 (1971). Turkova, Affinity Chromatography, J . C h r o m a t o g . 91, 267 (1974). R. & Dean, P . D . G . (1974) Affinity Chromatography, Wiley, London. (5) P . O ' C a r r a & S. B a r r y , Affinity Chromatography of lactate dehydrogenase. Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes, FEBS Letters 21, 281 (1972).
Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry by O. Hoffmann-Ostenhof